ABSTRACT
A Neurospora sp was used to ferment organic waste materials such as sugarcane residue powder (bagasse), bran and cassava residue The feed complex enzymes including cellulase, saccharifying enzyme,neutral proteinase and other growth factors were produced The experimental results showed that suitable medium contained sugarcane residue powder 100 g ?bran 600 g, cassava residue 300 g, mixed nitrogen sucrose 20 g per kilogram solid materials and some content of phosphate, the water in medium was twice as weight as the solid materials;the suitable fermentation conditions were that the strain was cultured first for 2 days under 34℃ and then fermented for 2～3 days at 37℃, no light,keeping the watering medium and suppling enough oxygen during fermentation The cellulase (CMC+C1) was 1759mg/g/h, saccharifying enzyme to 3110 mg/g/h, neutral proteinase 297mg/g /h in ferment product On the other hand, light,enough air,lower temperature and less water content in medium were benefit to producing spores
ABSTRACT
The cellulase production and extraction from Trichoderma Longibrachiatum ANU_3-958 were reported. The results indicated that maximum enzyme activity can be obtained when the fungus was grown on a solid medium for 144 hours, the proportion between the solid culture and the extracting agent was about 1:7(W/V) and extract was precipitated twice by adding solid (NH_4)_2SO_4. Cellulase powder obtained by freeze drying was analysed for its related enzyme activities. The highest CMC and FPA enzyme activity are about 2788.89IU/g enzyme powder and 79.44IU/g enzyme powder respectively. The yield for the solid culture is about 13.28% averagely.